Table 2: Temperature (°C) Distance (cm) Time (s) Rate of Reaction (cm/s) 10.0 8.00 5.85 1.38 21.0 8.00 4.83 1.66 35.0 8.00 2.99 2.68 50.0 8.00 4.21 1.90 80.0 8.00 5.52 1.45 Graph 2: Analysis 2: Observation graph 2 shows the relationship between the environmental temperature and the rate of reaction.
According to the observation chart the optimal temperature was 35°C.
Table 1: Enzyme concentration & compositions Distance (cm) Time (s) Rate of Change (cm/s) Other observations 100 % concentration (10 m L potato juice) 8 cm 3.02 s 2.65 cm/s – bubbles appeared 80 % concentration (8 m L potato juice, 2 m L distilled water) 8 cm 5.06 s 1.58 cm/s – fewer bubbles than previous composition 60 % concentration (6 m L potato juice, 4 m L distilled water) 8 cm 6.28 s 1.27 cm/s – fewer bubbles than previous composition 40% concentration (4 m L potato juice, 6 m L distilled water) 8 cm 7.5 s 1.07 cm/s – fewer bubbles than previous composition 20% concentration (2 m L potato juice, 8 m L distilled water) 8 cm 19.65 s 0.41 cm/s – no bubbles appeared Graph 1: Analysis 1: According to the observation graph 1, the major trend shows that as the concentration of the catalase, which is in the potato juice, increases there is also an increase in the rate of reaction.
As the concentration of the catalase decreased, the rate of reaction also decreased.
Factors include: concentration of the enzyme, temperature, p H level, concentration of the substrate, and inhibitors.
Enzymes Temperature Coursework Examples Of Critical Thinking Questions For Students
This lab shows the affects these factors have on the rate of reaction between catalase, an enzyme found in potatoes, and hydrogen peroxide, the substrate.Catalase can help break down other harmful chemicals in the body such as alcohol, phenol, and formaldehyde (Vitamin Stuff.com).As mentioned before, enzymes play a significant role in organic chemistry.The lower the activation energy is, the faster the rate of reaction is, and therefore enzymes speed up reactions in the body by lowering the activation energy required.(Diet-Health.net) There are many factors that contribute to the rate of reaction of an enzyme.Table 3: Amount of H2O2 (m L) Amount of Distilled Water (m L) Amount of p H Buffer (m L) p H Level Vertical Distance Travelled by Filter Paper Towards Meniscus Time taken by filter paper disc to move to meniscus (s) Upward velocity of Filter Paper Disc (cm/s) 10 m L 5 m L 7 (Control) 8.15 6.6 1.23 10 m L 5 m L 2 7.98.15 16.65 0.47 10 m L 5 m L 4 8.15 7.05 1.16 10 m L 5 m L 9 8.1 10.4 0.78 10 m L 5 m L 12 7.85 8.14 0.96 Graph 3: Analysis 3: According to graph 3, the optimal value was the p H level of 7.At the p H level of 7, the rate of reaction was the fastest, any p H level higher or lower than that of 7 the enzyme’s rate of reaction would decrease.Hydrogen peroxide also forms when white blood cells break down and kill bacteria in the body.Catalase is also helpful in prevent the formation of carbon dioxide bubbles in the blood.The specific enzyme that was studied during this lab was catalase.Catalase is a naturally occurring enzyme that is found in many living organisms such as plants and the human body.